The Effect of Transfection on Metastatic Potential of Mouse 3T3 cells


by : 
Ananya M.


I will be testing the variance of Metastatic Potential on Mouse 3T3 cells after they have been transfected. One group will be the Wild Type in which no transfection has been done. In another group, the p53 gene will be knocked down. The last group will have the Myosin Heavy Chain 9 gene will be knocked down. By using the Boyden Chamber, I will record the migration patterns of the cells, and I will observe the effects of transfection on the Metastatic Potential of these cells.